Transcription from chromatins of human lymphocytic leukemia cells and normal lymphocytes.

نویسندگان

  • H Sawada
  • V H Gilmore
  • G F Saunders
چکیده

Chromatins prepared from leukemic lymphosarcoma cell(s), (LS), acute lymphoblastic leukemia cells, chronic lymphocytic leukemia cells and normal tissue culture lymphocytes (NC-37) were studied with respect to their chemical compositions, thermal denaturation profiles, and template activities. Acute lymphoblastic leukemia and LS chromatins had approximately 2 to 4 times the nonhistone protein and RNA contents of CLL chromatin. NC-37 chromatin showed increased amounts of nonhistone protein and less histone content as compared with leukemic lymphocytes. LS chromatin had the highest template activity (10% of purified DNA). The template activities of acute lymphoblastic leukemia chromatin (8%) and CLL chromatin (5%) were also higher than those of NC-37 chromatin (4%). By hybridization competition experiments, it was shown that the RNA products synthesized from acute lymphoblastic leukemia chromatin had additional RNA species which were not present in the transcripts of CLL and NC-37 chromatins. In contrast, the RNA products from NC-37 chromatin were similar to those from leukemic lymphocyte chromatins (acute lymphoblastic leukemia cell and CLL). The results also showed that leukemic lymphocyte chromatins, especially from LS and acute lymphoblastic leukemia had more transcribable repetitive DNA sequences than did NC-37 chromatin. The data suggest that transcription of additional families of repetitive DNA sequences accompany the transition to cancer in the hematopoietic system.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Analysis of HLA-G Gene Expression in B-Lymphocytes from Chronic Lymphocytic Leukemia Patients

The human leukocyte antigen G (HLA-G) molecule exhibits limited tissue distribution, low polymorphism and alternative splicings that generate seven HLA-G isoforms. HLA-G exerts multiple immunoregulatory functions. Recent studies indicate an ectopic up-regulation in tumor cells that may favor their escape from anti-tumor immune responses. This study it is an effort to clarify the presence of HLA...

متن کامل

Transcription from Chromatins of Human Lymphocytic Leukemia Cells and Normal Lymphocytes1

Chromatins prepared from leukemic lymphosarcoma cell(s), (LS), acute lymphoblastic leukemia cells, chronic lymphocytic leukemia cells and normal tissue culture lymphocytes (NC-37) were studied with respect to their chemical compositions, thermal denaturation profiles, and template activities. Acute lymphoblastic leukemia and LS chromatins had approximately 2 to 4 times the nonhistone protein an...

متن کامل

Blimp-1 Expression as an Exhaustion Transcription Factor in Chronic Lymphocytic Leukemia

Background: PPreviously, it was shown that exhausted CD4+ and CD8+ T cells in chronic lymphocytic leukemia (CLL) co-express the two immune-inhibitory receptors, Tim-3 and PD-1. Present study investigated the expression of Blimp-1, a transcription factor involved in T cell exhaustion, in patients with CLL. Materials and Methods: Peripheral blood mononuclear cells were collected from 25 untreate...

متن کامل

Comparison Cytotoxic Effects of Mangifera Indica L. and Juglans Regia Aqueous Extract on Human Chronic Lymphocytic Leukemia

Natural products isolated from plant sources are well known for their pharmacological potential in diversity of disease treatments such as inflammatory or cancer conditions. Mango (Mangifera indica L.) and Juglans regia are thought to be rich of functional phytochemicals. To elucidate the anticancer activity of Juglans regia (JR) and Mangifera indica L (MI) aqueous extract were investigated on ...

متن کامل

Comparison Cytotoxic Effects of Mangifera Indica L. and Juglans Regia Aqueous Extract on Human Chronic Lymphocytic Leukemia

Natural products isolated from plant sources are well known for their pharmacological potential in diversity of disease treatments such as inflammatory or cancer conditions. Mango (Mangifera indica L.) and Juglans regia are thought to be rich of functional phytochemicals. To elucidate the anticancer activity of Juglans regia (JR) and Mangifera indica L (MI) aqueous extract were investigated on ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Cancer research

دوره 33 2  شماره 

صفحات  -

تاریخ انتشار 1973